Multiplex real-time PCR has high sensitivity and specificity for the detection of invasive fungi, and is suitable for peripheral blood (serum, plasma), bronchoalveolar lavage fluid, cerebrospinal fluid and other body fluids. Fluorescent probe primers specifically bind to the fungal DNA fragment to be detected through base complementary pairing to ensure the specificity of amplification. Exponential amplification of fungal DNA fragments to be detected by PCR requires only a trace amount of DNA to get sufficient detection fluorescence signal, so it has ultra-high sensitivity.

PtNGS uses a large number of primers for the target pathogen to perform multiplex PCR amplification of nucleic acids in the sample, followed by simultaneous sequencing analysis of these amplification products by high-throughput sequencing. PtNGS amplifies the signal of amplified targets by PCR at the nucleic acid capture stage of pathogens, and then uses high-throughput sequencing to simultaneously detect the amplified products to realize the quantitative analysis of pathogens, which well combines the high sensitivity of PCR technology and the high specificity of high-throughput sequencing and becomes a detection technology with both sensitivity and specificity.

The detection range of this project is based on the reference genome database that can provide wide-area search and continuous update, including about 500,000 + strains of microbial reference genome, and the results displayed by the detection are mainly from the carefully organized microbial species database capable of precise identification, 19,124 kinds in total, 9241 kinds of bacteria, 1,293 kinds of fungus, 8069 kinds of viruses, 327 kinds of parasites, 129 kinds of mycobacteria, 194 kinds of mycoplasmas/chlamydia/rickettsiae/ehrlich, etc.